Pathology


Our pathology department quickly provides high-quality histopathological specimens for our clients. We utilize a wealth of background knowledge and experience in toxicologic pathology to deliver accurate diagnoses.

 

The members of our pathology department include licensed study pathologists, necropsy technicians, and specimen preparation specialists with the following services:

  • Japanese Society of Toxicologic Pathology (JSTP)-certified toxicological pathologists
  • Japanese College of Veterinary Pathologists (JCVP)-certified veterinary pathologists
  • PhD holders in medical science or veterinary medicine
  • Qualified laboratory technologists in experimental pathology

The pathology department constantly strives to offer new services (non-decalcified grind specimen preparation, tissue cross-reactivity (TCR) study, virtual imaging of tissue slides, etc.) to best suit our client's needs.

Fingers of mature cynomolgus monkey (slide: 55 × 90 mm)
Fingers of mature cynomolgus monkey (slide: 55 × 90 mm)
Eyeball of a mature cynomolgus monkey (HE staining)
Eyeball of a mature cynomolgus monkey (HE staining)
Nasal cavity and canine tooth of a mature cynomolgus monkey (slide: 55 × 90 mm)
Nasal cavity and canine tooth of a mature cynomolgus monkey (slide: 55 × 90 mm)

Necropsy

Our experience in animal dissection is extensive. Our capabilities include but are not limited to dissection of mice, rats, rabbits, dogs, cynomolgus monkeys, pigs, guinea pigs, hamsters, and ferrets. We are especially proficient in the necropsy of cynomolgus monkeys. 

 

Preparation of Pathological Specimens

We prepare specimens from each animal for light microscopic and electron microscopic (transmission, scanning) examinations under the direction of our board-certified pathologists. Please contact us to discuss any special requests for specimen preparation. We like a challenge, which is why we have been able to prepare top class specimens for difficult tissues such as teeth and eyeballs.

 

Preparation for Light Microscopic Specimens

  • Trimming
  • Embedding
  • Sectioning
  • Staining(HE staining, special staining, immunohistochemical staining)

Preparation for Electron Microscopic Specimens (transmission)

  • Sampling and trimming
  • Resin embedding
  • Semi-ultra-thin sectioning
  • Ultra-thin sectioning
  • Electron staining
  • Electron microscopy (*) and imaging*: A tamper-prevention system for digital images is used with a digital camera on our transmission electron microscope to prepare images which can be submitted to the Japanese PMDA.

The specimens listed above (prepared for light or electron microscopic specimens) can be archived at our facilities.

 

Papillae of the tongue of the mature cynomolgus (HE staining)
Papillae of the tongue of the mature cynomolgus (HE staining)
Foliate papillae of the mature cynomolgus monkeys (SEM Observation)
Foliate papillae of the mature cynomolgus monkeys (SEM Observation)
Taste buds of circumvallate papillae of the mature cynomolgus (TEM observation)
Taste buds of circumvallate papillae of the mature cynomolgus (TEM observation)

Histopathology

Our highly qualified Japanese board-certified pathologists specialize in toxicologic pathology to ensure that you get the best information possible about your compound. Peer review has become more and more important in recent years, and we can recommend third party peer reviewers or support a visit from your in-house pathologist for peer review.  

 

Other

Non-decalcified specimen preparation

We embed hard tissues or samples (bones or teeth after fixation, dental equipment, or medical devices) in resin, and prepare thin sections using grinding and staining.

Tissue Cross-Reactivity (TCR)

TCR studies are recommended as a part of safety evaluation prior to preliminary clinical trials during antibody drug development. We accept and perform TCR studies in confidence, using human tissues, or tissues from various animals including cynomolgus monkeys.

View slides from anywhere in the world

We can easily scan histopathological glass slides and convert them into digital slides for your convenience using the Aperio digital slide system (Aperio AT2, Leica Biosystems).  Digital slides can be viewed online anywhere and at any time which can reduce overall cost by omitting the need for specimen transportation.

 

Selling Specimens

Frozen Tissues

We collect a variety of animal tissues and store them under humidity and temperature-controlled conditions. Samples are immediately frozen and sent to our clients on dry ice by request. For details, please feel free to contact us.

Normal Tissues

We provide various animal tissue specimens (HE staining), or corresponding digital slides. Please contact us for more information.